MultiMACS Cell24 Separator Plus from Miltenyi Biotec

Product Specs
ItemMultiMACS Cell24 Separator Plus
CompanyMiltenyi Biotec
Price Get Pricing Get Quote for this Product
Catalog Number130-098-637
ApplicationsCell separation
QuantityEA
Absorbance / Excitationn/a (magnetic)
Throughputup to 24 samples/run
Sample Volume5 mL, 24-well
Analysis TimeInquire
Flow RateInquire
DimensionsInquire
Regulatory StatusFor research use only
ComponentsMultiMACS 5 mL Tube Rack : Multi-24 Column Block : Power cord : MultiMACS Cell24 Separator : 2x 24-well Deep Well Plate : MACS Elution Station : Elution Chamber Base : Elution Chamber Lid I : Elution Chamber Lid II : Single-Column Adapter : Tubing : Column Blank Holder and 9 Column Blanks : Null modem cable : High power CAN cable

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Local Office

Miltenyi Biotec

Miltenyi Biotec Inc.

2303 Lindbergh Street

Auburn, CA 95602

United States

Phone: Phone: +1 530 888 8871
Toll Free: 800 FOR MACS
Fax: +1 530 888 8925

Email: macs@miltenyibiotec.com

Company Profile

Website: www.miltenyibiotec.com

International Office

Miltenyi Biotec

Miltenyi Biotec B.V. & Co. KG

Friedrich-Ebert-Straße 68

51429 Bergisch Gladbach

Germany

Phone: +49 2204 8306-0
Fax: +49 2204 8306-235

Email: macs@miltenyibiotec.de

Company Profile

Website: www.miltenyibiotec.com

(1) The coenzyme A precursor pantethine enhances antitumor immunity in sarcomaLife Science AllianceDecember 1, 2023Richard Miallot, Virginie Millet, Anais Roger, Romain Fenouil, Catherine Tardivel, Jean-Charles Martin, Laetitia Shintu, Paul Berchard, Juliane Sousa Lanza, Bernard Malissen, et al.cells, CD8a+ naïve T cells, and CD8+ TILs were isolated using microbeads from Miltenyi Biotech and MultiMACS Cell24 separation on LS columns according to the manufacturer’s protocols. The inguinal TDLNs were collected

(2) Human Dendritic Cell Subset Isolation by Magnetic Bead Sorting: A Protocol to Efficiently Obtain Pure PopulationsBIO-PROTOCOLOctober 20, 2023Georgina Flórez-Grau, Jorge Cuenca Cuenca Escalona, Helena Lacasta-Mambo, Daphne Roelofs, Johanna Bödder, Ruben Beuk, Gerty Schreibelt, Jolanda I. M. de Vriesavailable) can also be used. Equipment MultiMACS Cell24 Separator Plus (Miltenyi Biotec, catalog number: 130-098-637)QuadroMACS Separator (Miltenyi Biotec, catalog number: 130-090-976)OctoMACS Separator (Miltenyi Biotec,

(3) Extracellular Matrix Expression in Human Pancreatic Fat Cells of Patients with Normal Glucose Regulation, Prediabetes and Type 2 DiabetesInternational Journal of Molecular SciencesJuly 6, 2023Dorothea Siegel-Axel, Morgana Barroso Oquendo, Felicia Gerst, Falko Fend, Robert Wagner, Martin Heni, Alfred Königsrainer, Hans-Ulrich Häring, Andreas Fritsche, Erwin Schleicher, et al.of CD14+ cells directly from anticoagulated whole blood [69] using the autoMACS Pro Separator, the MultiMACS Cell24 Separator Plus (Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany), or the Whole Blood Column Kit (Whole Blood

(4) An OMA1 redox site controls mitochondrial homeostasis, sarcoma growth, and immunogenicityLife Science AllianceJune 1, 2023Richard Miallot, Virginie Millet, Yann Groult, Angelika Modelska, Lydie Crescence, Sandrine Roulland, Sandrine Henri, Bernard Malissen, Nicolas Brouilly, Laurence Panicot-Dubois, et al.Alternatively, CD45-negative cells were isolated using CD45 microbeads from Miltenyi Biotec and the MultiMACS Cell24 separation on LS columns according to the manufacturer’s protocols. Analysis of OMA1 and OPA1 expressions

(5) Simultaneous isolation of CD45 tumor-infiltrating lymphocytes, tumor cells, and associated fibroblasts from murine breast tumor model by MACSSTAR ProtocolsMarch 1, 2023Laura Kalfeist, Stacy Petit, Loïck Galland, François Ghiringhelli, Sylvain Ladoire, Emeric LimagneSoftware,https://www.graphpad.com/scientific-software/prism/ ,, Other,Other,Other ,, Isoflurane (Vetflurane®),Virbac,N/A The MultiMACS™ Cell24 Separator Plus,MIltenyi Biotec,Cat# 130-098-637 gentleMACS Dissociator,MIltenyi Biotec,Cat# 130-093-235 "CytoFLEX V5-B5-R3 Flow Cytometer (13 Detectors,

(6) The Appendix Orchestrates T-Cell Mediated Immunosurveillance in Colitis-Associated CancerCellular and Molecular Gastroenterology and HepatologyJanuary 1, 2023Maxime K. Collard, Julien Tourneur-Marsille, Mathieu Uzzan, Miguel Albuquerque, Maryline Roy, Anne Dumay, Jean-Noël Freund, Jean-Pierre Hugot, Nathalie Guedj, Xavier Treton, et al.the manufacturer’s instructions. Magnetic separation was performed with the MultiMACs Separator Plus (130-098-637; Miltenyi). Flow Cytometry Cell suspensions were Fc-blocked (FcR Blocking mouse, 130-092-575; Miltenyi),

(7) Transient regulation of focal adhesion via Tensin3 is required for nascent oligodendrocyte differentiationeLifeOctober 10, 2022Emeric Merour, Hatem Hmidan, Corentine Marie, Pierre-Henri Helou, Haiyang Lu, Antoine Potel, Jean-Baptiste Hure, Adrien Clavairoly, Yi Ping Shih, Salman Goudarzi, et al.centrifugation, leaving bound cells that were sorted using MultiMACS Cell24 Separator Plus (Miltenyi Biotec; ref 130-098-637). Sorted cells were either plated in culture plates for in vitro cell study or centrifuged at 1200 rpm

(8) Intestinal single-cell atlas reveals novel lymphocytes in pigs with similarities to human cellsLife science allianceOctober 1, 2022Wiarda JE, Trachsel JM, Sivasankaran SK, Tuggle CK, Loving CL.used for the divided samples to facilitate magnetic sorting using a MultiMACS Cell24 Separator Plus (130-098-637; Miltenyi). LS columns were prerinsed with 1× Binding Buffer before applying cells. Columns were rinsed

(9) Salt Transiently Inhibits Mitochondrial Energetics in Mononuclear PhagocytesCirculationJuly 13, 2021Geisberger S, Bartolomaeus H, Neubert P, Willebrand R, Zasada C, Bartolomaeus T, McParland V, Swinnen D, Geuzens A, Maifeld A, et al.Monocyte isolation was performed using the Pan Monocyte Isolation Kit human (130-096-537, Miltenyi Biotec) and the MultiMACS Cell24 Separator Plus (Miltenyi Biotec) according to the manufacturer’s instructions. Purity of monocyte enrichment was determined

(10) Epigenetic silencing by SETDB1 suppresses tumour intrinsic immunogenicityNatureJuly 1, 2021Griffin GK, Wu J, Iracheta-Vellve A, Patti JC, Hsu J, Davis T, Dele-Oni D, Du PP, Halawi AG, Ishizuka JJ, et al.manufacturer’s instructions. Enrichment for CD45+ cells was performed using magnetic beads (Miltenyi) and the MultiMACS Cell24 Separator Plus (Miltenyi). Cells were then stained in PBS using Live/Dead Fixable Near-IR Dead Cell Stain Kit (1:100,

(11) Lentiviral delivery of combinatorial CAR/CRISPRi circuit into human primary T cells is enhanced by TBK1/IKKɛ complex inhibitor BX795Journal of translational medicineSeptember 23, 2020Li L, Gao Y, Srivastava R, Wang W, Xiong Q, Fang Z, Pelayo A, Denson C, Goswami A, Harari-Steinfeld R, et al.positive selection using the S traightFrom® Leukopak® CD4/ CD8 MicroBead kit, human (Miltenyi Biotec) and MultiMACS™ Cell24 Separator Plus (Miltenyi Biotec) according to the manufacturer’s instructions. Lentiviral transduction of T cells On

(12) The Quantitative Assessment of the Secreted IgG Repertoire after Recall to Evaluate the Quality of ImmunizationsThe Journal of ImmunologyAugust 15, 2020Klaus Eyer, Carlos Castrillon, Guilhem Chenon, JÃ©rÃ´me Bibette, Pierre Bruhns, Andrew D. Griffiths, Jean Baudryprocessed according to the manufacturer’s protocol using the Pan B Cell Isolation Kit II (Miltenyi) on a MultiMACS Cell24 Separator Plus (program depletion; Miltenyi). Purity of B cell lineage was usually above 90% (data not shown). Titer

(13) Quantitative modeling of the effect of antigen dosage on B-cell affinity distributions in maturating germinal centerseLifeJune 15, 2020Marco Molari, Klaus Eyer, Jean Baudry, Simona Cocco, RÃ©mi Monassonprocessed according to the manufacturer’s protocol using the Pan B Cell Isolation Kit II (Miltenyi) on a MultiMACS Cell24 Separator Plus (Miltenyi, program depletion). Purity of B-cell lineage was usually above 90% (data not shown). Antigen

(14) LIF regulates CXCL9 in tumor-associated macrophages and prevents CD8⁺ T cell tumor-infiltration impairing anti-PD1 therapyNature communicationsJune 11, 2019Pascual-García M, Bonfill-Teixidor E, Planas-Rigol E, Rubio-Perez C, Iurlaro R, Arias A, Cuartas I, Sala-Hojman A, Escudero L, Martínez-Ricarte F, et al.digested with Tumor Dissociation kit and CD11b+ cells were isolated using CD11b magnetic beads and the MultiMACS Cell24 separator Plus (all from Miltenyi Biotec). CD11b+ cells obtained were cultured in RPMI medium supplemented with 10%

(15) Oligodendrocyte precursor survival and differentiation requires chromatin remodeling by Chd7 and Chd8Proceedings of the National Academy of SciencesAugust 28, 2018Corentine Marie et al.sorting was done using anti-O4–coupled-beads (130-094-543) and the MultiMACS Cell24 Separator Plus (130-098-637). O4+-cells were collected in PBS before being process to RNA-seq, ATAC-seq or ChIP-seq. ChIP-Seq,

(16) Bim suppresses the development of SLE by limiting myeloid inflammatory responsesThe Journal of experimental medicineDecember 4, 2017Tsai F, Homan PJ, Agrawal H, Misharin AV, Abdala-Valencia H, Haines GK 3rd, Dominguez S, Bloomfield CL, Saber R, Chang A, et al.(BD Biosciences). Isolated kidney cells were labeled with CD45 MicroBeads (Miltenyi Biotec) and separated on MultiMACS Cell24 Separator Plus (Miltenyi Biotec). Total numbers of live cells were counted by Countess Automated cell counter (Invitrogen),

(17) High-Throughput Single-Cell Derived Sphere Formation for Cancer Stem-Like Cell Identification and AnalysisScientific ReportsJuly 1, 2016Yu-Chih Chen, Patrick N. Ingram, Shamileh Fouladdel, Sean P. McDermott, Ebrahim Azizi, Max S. Wicha, Euisik Yoonincubated 15 min in the refrigerator. While incubating, a LS column (Miltenyi Biotec) was equilibrated on a MultiMACS Cell24 Separator Plus (Miltenyi Biotec) with 3 × 1000 μL PBS/0.5% BSA. Following 15 min incubation, 400 μL PBS/0.5% BSA was

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